1. Induction of apoptosis by NMT siRNAs.
Induction of apoptosis by NMT siRNAs. SK-OV-3 cells were treated with 20 nmol/L negative control siRNA (A), 10 nmol/L NMT1-1 siRNA (B), 10 nmol/L NMT2-4 siRNA (C), or 10 nmol/L each of NMT1-1 and NMT2-4 siRNA (D) for 48 hours as described in Materials and Methods and then scored for apoptosis using the TdT-mediated dUTP nick end labeling assay. Panels are matched sets of bright field (bottom) and fluorescent (TdT-mediated dUTP nick end labeling positive; top) images for each treatment. E. Graphical representation of the percentage of apoptotic cells in each treatment condition. Negative control–treated cells had an average of 1.0 ± 0.3% of apoptotic cells. NMT11-treated cells had an average of 7.8 ± 1.1% of apoptotic cells. NMT2-4-treated cells had an average of 19.0 ± 2.0% of apoptotic cells. NMT1-1- and NMT2-4-treated cells had an average of 29.8 ± 2.0% of apoptotic cells.
Charles E. Ducker et al. Mol Cancer Res 2005;3:
©2005 by American Association for Cancer Research