1. siRNA directed at Ngn1 inhibited differentiation of inner ear stem cells to β-III tubulin-positive cells. a, Inner ear stem cells treated with siRNA to Ngn1 compared with nontargeting siRNA (red) had a decreased percentage of TuJ-positive cells (green).
siRNA directed at Ngn1 inhibited differentiation of inner ear stem cells to β-III tubulin-positive cells. a, Inner ear stem cells treated with siRNA to Ngn1 compared with nontargeting siRNA (red) had a decreased percentage of TuJ-positive cells (green). Blue, DAPI. Scale bar, 20 μm. b, Ngn1 siRNA inhibited differentiation to β-III tubulin-positive cells. c, NICD-transfected cells were treated with siRNA, and differentiation to a neuronal morphology was unaffected by a nontargeting sequence (β-III tubulin-positive cells shown in blue), but their expression of β-III tubulin was blocked by siRNA to Ngn1. d, Cells that expressed nuclear GFP were counted for β-III tubulin after siRNA treatment. Error bar, SE. *p < 0.05, significant difference.
Sang-Jun Jeon et al. J. Neurosci. 2011;31:
©2011 by Society for Neuroscience