1. Assay of ROS accumulation in cells exposed to high levels of glucose.
Assay of ROS accumulation in cells exposed to high levels of glucose. Cells exposed to 5.5 mmol/l as control or high levels of glucose such as 22 and 33 mmol/l glucose for varying time periods as indicated were subjected to carboxyl-H2-DCFDA exposure for 1 h before the end of each exposure. Each culture was first checked by a phase-contrast microscope to ensure that the cell density among the cultures were comparable. The fluorescence was then detected by a confocal microscope as shown. The staining intensity thus corresponds to the extent of ROS accumulation. Original magnification ×63.
Lu Cai et al. Diabetes 2002;51:
©2002 by American Diabetes Association