1. Identification of a Commonly Used CDR3 Region of Infiltrating T Cells Expressing Vβ13 and Vβ15 Derived From Psoriasis Patients 
Ha Young Hwang, Young Yil Bahk, Tae-Yoon Kim 
Journal of Investigative Dermatology 
Volume 120, Issue 3, Pages (March 2003)
DOI: /j x
Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

2. Figure 1
RT-PCR analysis of expression of TCR β-chain. Total RNA from skin biopsies from six psoriasis patients was reverse transcribed to cDNA. cDNA, which was normalized with GAPDH, was used for the first round PCR with Vβ-specific primer and Cβ3 primer in a total of 23 cycles. The second round PCR with 0.5 μl of the first round PCR product, Vβ-specific primer, and Cβ5 primer (internal primer of constant region) was carried out for 25 cycles. The PCR products were analyzed on a 1.3% agarose gel. N means nonlesional skin and L means lesional skin from psoriasis patients. Arrows in right size indicate the real PCR products.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

3. Figure 2
Detection of the CDR3 motif (Vβ13-DWTSGV-Jβ2.7) in skin biopsies derived from seven psoriasis patients (patients 7-13) and six healthy individuals (H1-H8). cDNA was prepared from total RNA from 2mm skin biopsies by reverse transcription and normalized with GAPDH. Normalized cDNA was first amplified using Vβ13 primer and Cβ3 primer. The second round PCR was conducted using oligonucleotide corresponding to DWTSGV-Jβ2.7 and Cβ3 for CDR3, and Cβ2 and Cβ3 primer for the constant region as internal control, respectively. The PCR products were separated on a 1.3% agarose gel and transferred to a positively charged nylon membrane and covalently fixed by ultraviolet light. Then, the membrane was prehybridized, and hybridized with 32P-radiolabeled oligonucleotide probe corresponding to the Vβ13-DWTSGV-Jβ2.7 at 56°C and Cβ5 specific for the constant region at 44°C. After washing, the membrane was analyzed after exposure on X-ray film.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

4. Figure 3
Detection of the CDR3 motif Vβ13-DWTSGV-Jβ2.7 in skin biopsy specimens of nonlesional skin (N) and psoriatic lesional skin (L) derived from psoriasis patients. Skin biopsies obtained from 15 psoriasis patients (patients 14-28) were analyzed under the same conditions as described in Fig 2.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

5. Figure 4
Detection of the CDR3 motif (Vβ13-DWTSGV-Jβ2.7) in PBMC and in CLA+ cells from psoriasis patients and healthy individuals. PBMC was isolated from heparinized blood by density centrifugation using Ficoll-Hypaque. CLA+ cells were purified from PBMC by the magnetic cell separation method as described in Materials and Methods. The existence of the specific CDR3 motif in PBMC obtained from seven patients with psoriasis (patients 22-28) and three healthy individuals (H1-H3) was analyzed, and in CLA+ cells from five patients with psoriasis (patients 29-33) and three healthy individuals (H4-H6) as previously mentioned. NC means negative control and PC means positive control. (A) Detection of the CDR3 motif in PBMC and (B) detection of the CDR3 motif in CLA+ cells.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions