1. The B. thetaiotaomicron OMV pellet (OMVp) protein content is different from that of the OM. Three hundred micrograms of each preparation and biological replicates of purified OM and OMVp proteins were digested with trypsin.
The B. thetaiotaomicron OMV pellet (OMVp) protein content is different from that of the OM. Three hundred micrograms of each preparation and biological replicates of purified OM and OMVp proteins were digested with trypsin. The resulting peptides were enriched and then analyzed via liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) as explained in Materials and Methods, followed by protein identification with the Mascot search engine using the UniProt database. Volcano plot shows OM and OMVp protein populations. Red labels indicate the proteins with the highest OMVp enrichment in comparison to that of OM.
Ezequiel Valguarnera et al. mSphere 2018; doi: /mSphere