1. Engagement of PDGF-αR and PDGF-βR is critical for PDGF-BB-mediated increased proliferation of NPCs. RT-PCR (A) and WB (B) of PDGF-αR and PDGF-βR in NPCs; M denotes DNA marker.
Engagement of PDGF-αR and PDGF-βR is critical for PDGF-BB-mediated increased proliferation of NPCs. RT-PCR (A) and WB (B) of PDGF-αR and PDGF-βR in NPCs; M denotes DNA marker. C, Immunostaining of PDGF-αR and PDGF-βR (red), which colocalized with nestin-positive staining (green) and DAPI (blue). Scale bars, 5 μm. D, PDGF induced PDGF-αR and PDGF-βR phosphorylation as determined by immunoprecipitation. E, STI-571 abolished PDGF-BB-mediated increase in proliferation. The data are presented as mean ± SD of four individual experiments. *p < 0.05 versus control group; ##p < 0.01 versus cocaine–Tat-treated group; +p < 0.05 versus both PDGF-BB-treated and Tat–cocaine-treated groups. F, WB analysis of whole-cell lysates from NPCs transfected with siRNAs PDGF-αR and PDGF-βR or nonsense (Non) siRNA, using antibodies specific for PDGF-αR and PDGF-βR. Data are representative of three independent experiments. G, Transfection of NPCs with siRNAs specific for PDGF-αR/PDGF-βR or nonsense siRNA abolished PDGF-BB-mediated increase of NPC proliferation. The data are presented as mean ± SD of four individual experiments. ***p < versus control group; #p < 0.05 versus PDGF-BB-treated cells transfected with nonsense siRNA group.
Honghong Yao et al. J. Neurosci. 2012;32:
©2012 by Society for Neuroscience