1. A. baumannii activates the autophagosome-lysosome system.
A. baumannii activates the autophagosome-lysosome system. (A and B) Additive effect of autophagy and TFEB on A. baumannii internalization by A549 cells. A549 cells were transfected with scrambled (SC) or TFEB siRNA or pEGFP-N1-TFEB and treated with pepstatin (20 µg/ml), bafilomycin (0.8 µM), or wortmannin (1 µM), and infected with 108 CFU/ml A. baumannii ATCC for 2 h to study bacterial adherence and invasion to host cells. Results are representative of three independent experiments, and data are the means plus SEM. Values for treated and untreated groups that are significantly different (P < 0.05): are indicated by an asterisk. CTL, control.
Raquel Parra-Millán et al. mSphere 2018; doi: /mSphere