1. Effect of AedaeCAPA-1 on in vitro fluid secretion rate, cation (Na+ and K+) concentration and transport rate by adult female A. aegypti MTs stimulated with a variety of diuretic hormones.
Effect of AedaeCAPA-1 on in vitro fluid secretion rate, cation (Na+ and K+) concentration and transport rate by adult female A. aegypti MTs stimulated with a variety of diuretic hormones.AedaeCAPA-1 (1 fmol l−1) was applied to MTs stimulated with 100 nmol l−1 5-HT, 10 nmol l−1RhoprDH (CRF), 50 nmol l−1 CDP or 25 nmol l−1DromeDH31 (DH31). (A) The tubule secretion assay was performed over a 60 min incubation period for the diuretic and AedaeCAPA-1, and 120 min for unstimulated controls. (B) Na+ and (C) K+ concentrations in the secreted fluid were measured using ion-selective microelectrodes (ISME), and the values were used to calculate the cation transport rate and ratio (D–F). Values are presented as means±s.e.m., n=20–63. Bars that are not significantly different from unstimulated controls are denoted with the same letter, as determined by a one-way ANOVA and Bonferroni post-test. Statistical significance between the two experimental treatments involving a specific diuretic factor alone or in combination with AedaeCAPA-1 is denoted by an asterisk, as determined by an unpaired t-test.
Farwa Sajadi et al. J Exp Biol 2018;221:jeb177089
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