1. MSK1 is necessary for BDNF-dependent CREB phosphorylation in area CA1.
MSK1 is necessary for BDNF-dependent CREB phosphorylation in area CA1. A, Treatment of wild-type hippocampal slices (upper panels) with BDNF (50 ng/ml; 10 min) resulted in a robust increase in CREB phosphorylation in CA1 neurons, compared to control untreated slices, as indicated by changes in immunofluorescence associated with a monoclonal antibody directed at the phosphorylation of CREB S133. In contrast, BDNF induced little or no effect on CREB phosphorylation in slices taken from MSK1 KD mice (lower panels). B, Box and whisker plot showing mean (open square), median, 25 and 75 percentile (box), and ±1 standard deviation (whiskers) of data from 21 BDNF-treated slices from eight wild-type mice and 20 BDNF-treated slices from 10 MSK1 KD mice. The specificity of the antibody for the phosphorylation of CREB S133 in CA1 pyramidal neurons was confirmed using a mouse expressing a CREB S133-alanine point mutation (data not shown).
Stephanie Daumas et al. eNeuro 2017;4:ENEURO
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