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Immunofluorescence analyses of untreated (A,C,E) and tamoxifen-treated (B,D,F) Cx43fl/fl:SM-CreERT2 uterus. Immunofluorescence analyses of untreated (A,C,E)

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Presentation on theme: "Immunofluorescence analyses of untreated (A,C,E) and tamoxifen-treated (B,D,F) Cx43fl/fl:SM-CreERT2 uterus. Immunofluorescence analyses of untreated (A,C,E)"— Presentation transcript:

1 Immunofluorescence analyses of untreated (A,C,E) and tamoxifen-treated (B,D,F) Cx43fl/fl:SM-CreERT2 uterus. Immunofluorescence analyses of untreated (A,C,E) and tamoxifen-treated (B,D,F) Cx43fl/fl:SM-CreERT2 uterus. (A,B) Cryosections of uterine tissue were analysed using antibodies to Cx26 (red) and smooth muscle actin (green). Most of the signals were detected in the glandular epithelium (GE). (C,D) Immunofluorescence of Cx40 (red) and smooth muscle actin (green) in uterine cryosections revealed only sparse signals in myometrial SMCs but abundant staining of blood vessels (BV). (E,F) Staining of uterine tissue using antibodies to Cx45 (red) and smooth muscle actin (green) revealed extensive expression in myometrial smooth muscle cells. The colocalization of connexin and smooth muscle actin signals is visualized in yellow. BV, Blood vessel; CM, circular muscle layer; GE, glandular epithelium; LM, longitudinal muscle layer; S, stroma. Bar, 100 μm. Britta Döring et al. J Cell Sci 2006;119: © The Company of Biologists Limited 2006

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