1. Structure–function analysis of CGEF-1.
Structure–function analysis of CGEF-1. (A) Summary of fusion proteins analyzed. Endogenous regions of the protein are shaded blue (DH-PH domains) and black (remainder of protein), GFP is green, rat PHPLC1∂1 is magenta and HA is yellow. Localization of the fusion protein, and whether it can recruit PAR-6 to the cortex, is summarized. (B-D′) Localization of overexpressed indicated fusion protein and PAR-6 in co-stained embryos; GFP-PHPLC1∂1 was used as a negative control. (E) Quantification of PAR-6 asymmetry was performed as for Fig. 1B. ***P<0.001 relative to control GFP-PHPLC1∂1 using two-tailed Student's t-test; NS, not significant.
Emily Chan, and Jeremy Nance J Cell Sci 2013;126:
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