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Transplantation of cryopreserved muscle cells in dilated cardiomyopathy: Effects on left ventricular geometry and function  Nobuhisa Ohno, MD, Paul W.M.

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Presentation on theme: "Transplantation of cryopreserved muscle cells in dilated cardiomyopathy: Effects on left ventricular geometry and function  Nobuhisa Ohno, MD, Paul W.M."— Presentation transcript:

1 Transplantation of cryopreserved muscle cells in dilated cardiomyopathy: Effects on left ventricular geometry and function  Nobuhisa Ohno, MD, Paul W.M Fedak, MD, Richard D Weisel, MD, Donald A.G Mickle, MD, Takeshiro Fujii, MD, Ren-Ke Li, MD, PhD  The Journal of Thoracic and Cardiovascular Surgery  Volume 126, Issue 5, Pages (November 2003) DOI: /S (03)

2 Figure 1 Experimental design. A, differential in vitro effects of cryopreservation on muscle cell growth and morphology from myopathic versus normal donor hamsters. Open bars indicate cells from normal hamsters, and shaded bars indicate cells from cardiomyopathic BIO53.58 hamsters. B, In vivo effects of muscle cell transplantation from cardiomyopathic donors for use in dilated cardiomyopathy after prior cryopreservation. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /S (03) )

3 Figure 2 Photomicrographs of cultured VSMCs (A and B) and SKMCs (C and D) obtained from cryopreserved normal and cardiomyopathic hamsters. The normal spindle-shaped VSMCs contrasted in appearance with the cardiomyopathic VSMCS that were larger and not spindle shaped. The normal and cardiomyopathic SKMCs were similar in appearance. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /S (03) )

4 Figure 3 Photomicrographs of cultured cyropreserved BIO SKMCs (A; original magnification 100×) and VSMCs (B; original magnification 100×). The SKMCs stained positively for myoD, and the VSMCs stained positively for α-smooth muscle actin. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /S (03) )

5 Figure 4 Peak systolic, developed, and end-diastolic pressures of normal, SKMC, VSMC, and control hearts with increasing balloon volumes. The peak systolic and developed pressures of the SKMC and normal hearts were significantly higher than the pressures of the VSMC hearts, which were greater than those of the control hearts. Diastolic function was not significantly different. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /S (03) )

6 Figure 5 Location of bromodeoxyuridine-labeled SKMCs transplanted in the anterior left ventricle of the cardiomyopathic BIO hamster. The labeled cells (arrows) were identified only in the LV anterior wall. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /S (03) )

7 Figure 6 Hematoxylin and eosin–stained (HE), anti-bromodeoxyuridine–stained (BrdU), and elastica Masson trichrome–stained (EMT) SKMC and VSMC transplants in the host BIO hamster LV anterior wall. The SKMC transplant contained many BrdU-labeled cells (C), was substantial, and had less fibrosis and more elastin (A and E) than the VSMC transplant (B, D, and F). Some of the SKMCs had mature sarcomeres (inset, C). The VSMC transplant tended to be localized to the subepicardium, whereas the SKMC transplant could be found in the subepimyocardium and midmyocardium. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /S (03) )

8 Figure 7 Immunohistochemical staining of the SKMC and VSMC transplants. The SKMC transplant stained positively for muscle-specific actin (MSA; A) and skeletal muscle fast myosin heavy chain (SMHC; B) and negatively for β-myosin heavy chain (βMHC; C). The VSMC transplant stained positively for α-smooth muscle actin (αSMA; D). Arrows indicate positive staining. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /S (03) )

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