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Identification of IL-16 as the lymphocyte chemotactic activity in the bronchoalveolar lavage fluid of histamine-challenged asthmatic  patients  Margaret.

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Presentation on theme: "Identification of IL-16 as the lymphocyte chemotactic activity in the bronchoalveolar lavage fluid of histamine-challenged asthmatic  patients  Margaret."— Presentation transcript:

1 Identification of IL-16 as the lymphocyte chemotactic activity in the bronchoalveolar lavage fluid of histamine-challenged asthmatic  patients  Margaret Vallen Mashikian, MD, Robert E. Tarpy, MD, Jussi J. Saukkonen, MD, Kaiser G. Lim, MD, Gregg D. Fine, BS, William W. Cruikshank, PhD, David Center, MD  Journal of Allergy and Clinical Immunology  Volume 101, Issue 6, Pages (June 1998) DOI: /S (98) Copyright © 1998 Mosby, Inc. Terms and Conditions

2 Fig. 1 Mean values for lymphocyte migration induced by concentrated BALF obtained from six normal subjects, six atopic nonasthmatic subjects, and eight asthmatic subjects after segmental challenge with saline or histamine. Cell migration is expressed as percentage of control cell migration in presence of PBS alone. BALF obtained 15 minutes after saline challenge is depicted in solid bars; 6 hours after saline challenge in shaded bars; 15 minutes after histamine challenge in open bars; and 6 hours after histamine challenge in striped bars. Asterisks denote cell migration induced by histamine-challenged BALF from asthmatic subjects at 6 hours, which was statistically different from migration induced by histamine-challenged BALF from normal subjects and atopic nonasthmatic subjects at 6 hours (p < 0.05). Pound sign (#) denotes cell migration induced by histamine-challenged BALF from asthmatic subjects at 6 hours, which was statistically different from migration induced by histamine-challenged BALF from asthmatic subjects at 15 minutes (p < 0.005). Journal of Allergy and Clinical Immunology  , DOI: ( /S (98) ) Copyright © 1998 Mosby, Inc. Terms and Conditions

3 Fig. 2 Lymphocyte migration induced by concentrated BALF obtained from six normal subjects (A), six atopic nonasthmatic subjects (B), or eight asthmatic subjects (C) after segmental challenge with saline or histamine. Cell migration is expressed as percentage of control cell migration in presence of PBS alone. For each subject, chemotactic activity in BALF obtained 15 minutes after saline challenge is depicted in solid bars; 6 hours after saline challenge in shaded bars; 15 minutes after histamine challenge in open bars; and 6 hours after histamine challenge in hatched bars. Asterisks denote statistically significant cell migration compared with control chemotactic buffer (p < 0.05). Journal of Allergy and Clinical Immunology  , DOI: ( /S (98) ) Copyright © 1998 Mosby, Inc. Terms and Conditions

4 Fig. 3 Effects of neutralizing anti–IL-16 antibody, anti–MIP-1α antibody, and IgG 2a isotype control antibody on BALF-induced T cell migration. BALF obtained from asthmatic subjects after saline and histamine challenge at 15 minutes and 6 hours was assessed in presence of anti–IL-16 antibody sufficient to neutralize 50 ng/ml of recombinant human IL-16 bioactivity. Samples were first assessed after 1:1 dilution of PBS (solid bars), then assessed 1:1 in presence of anti–IL-16 antibody (shaded bars). Induction of cell migration was then assessed for BALF obtained from six asthmatic subjects 6 hours after histamine challenge in presence of anti–MIP-1α antibody (open bar) and isotype control antibody (striped bar) sufficient to neutralize 50 ng/ml of bioactivity. Mean value for cell migration was calculated for each time point. Data are expressed as percentage of control cell migration in presence of PBS alone, with asterisks denoting inhibition of migration that was statistically different from BAL-induced migration in absence of blocking antibodies (p < 0.05) and pound sign denoting inhibition of migration that was statistically different from BAL-induced migration in presence of anti–MIP-1α and isotype control antibodies. Journal of Allergy and Clinical Immunology  , DOI: ( /S (98) ) Copyright © 1998 Mosby, Inc. Terms and Conditions

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