1. A: Gene expression of β-myosin heavy chain (hypertrophic marker) was analyzed on real-time PCR and normalized against 18S expression. n = 8–11 for each group.
A: Gene expression of β-myosin heavy chain (hypertrophic marker) was analyzed on real-time PCR and normalized against 18S expression. n = 8–11 for each group. B: Representative Northern blot showing total RNA. GAPDH was used to normalize for RNA loading (insert). Gene expression of ANP (n = 9–10 per group) and BNP (n = 4–5 per group) was assessed. Mean values for Ntg sham were normalized to 1. *P < 0.05 vs. Ntg sham; †P < 0.05 vs. Ntg diabetic. Sh, sham; D, diabetes.
Karina Huynh et al. Diabetes 2010;59:
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