1. (A) Correlation between the shear modulus (Fig
(A) Correlation between the shear modulus (Fig. 3B) and the density of vimentin intermediate filaments (green) or the density of membranes of the endoplasmic reticulum (ER) (red) as a function of the distance to the nucleus in RPE1 cells plated on...
(A) Correlation between the shear modulus (Fig. 3B) and the density of vimentin intermediate filaments (green) or the density of membranes of the endoplasmic reticulum (ER) (red) as a function of the distance to the nucleus in RPE1 cells plated on crossbow micropatterns. Cells were fixed and stained for vimentin using an anti-vimentin antibody or stained for ER membranes using a live-cell ER marker, and then fixed. Fluorescence intensities were quantified by averaging intensity profiles along 5-pixel-wide lines starting at the nucleus edge (n = 37 profiles from 15 cells for vimentin; n = 29 profiles from 16 cells for ER). The decrease of the shear modulus 1–2.5 µm away from the nucleus correlates with a decrease in vimentin intensity. (B) Images of the vimentin and ER stainings in typical cells (Left) and heat maps of the averaged vimentin and ER stainings (Right).
Kalpana Mandal et al. PNAS 2016;113:46:E7159-E7168
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