1. The Isotonix® Advantage
Mark Lange, Ph.D.
Director, Quality Control
Market America

2. OVERVIEW Understanding Your Digestive System
Transporting Nutrients into Cells
The Isotonix Delivery System
Isotonix Advantage #1,2,3…..

4. Just the thought of eating food starts the digestive process
Just the thought of eating food starts the digestive process. For example the salivary glands in our mouths are activated, producing an enzyme that will digest starch into simpler sugar molecules. An enzyme is a substance that speeds up a chemical reaction.

5. The large, hollow organs of the digestive tract contain a layer of muscle that enables their walls to move. The movement of organ walls can propel food and liquid through the system and also can mix the contents within each organ.
Food moves from one organ to the next through muscle action called peristalsis. Peristaliss looks like an ocean wave traveling through the muscle.
The muscle of the organ contracts to create a narrowing and then propels the narrowed portion slowly down the length of the organ. These waves of narrowing push the food and fluid in front of them through each hollow organ.

6. STOMACH Secretes 5 substances mainly: Water – diluent Chloride ions
Hydrogen ions
Pepsin – proteolytic enzyme (proteins)
Intrinsic factor – vitamin B-12 binding protein released by parietal cells
Hydrochloric acid
HCl = pH 1.0 – 2.8
Swallowed food is pushed into the esophagus, which connects the throat above with the stomach below. The stomach has three mechanical tasks.
First, it stores the swallowed food and liquid. It does this by opening and closing ringlike muscles, called sphincter muscles, at the top and bottom of the stomach.
Second, it mixes up the food, liquid, and digestive juice produced by the stomach.
The third task of the stomach is to slowly empty its contents into the small intestine.

9. Isotonix Advantage #1
Nutrients are not exposed to acidic environment of stomach, where they would otherwise degrade.
Customer money is not wasted.

10. Isotonix Advantage #2 Reduced delivery time.
Example: B vitamins for energy
Minutes to uptake – not hours

11. SMALL INTESTINE
ACIDIC FOOD SOLUTION stimulates pancreas to release numerous digestive enzymes in a sodium bicarbonate solution
NEUTRALIZATION pH of food solution changed from acidic to nearly neutral
After the stomach empties the food and juice mixture, called chime, into the small intestine, the juices of two other digestive organs mix with the food.
One of these organs, the pancreas, produces a juice that contains a wide array of enzymes to break down the carbohydrate, fat, and protein in food.
A base called sodium bicarbonate is also released to neutralize the acid originating from the stomach.

12. SMALL INTESTINE
BILE is a product of the liver, collected in the gall bladder, and secreted onto the food solution.
Contains surfactants that disperse fat.
Lipases act on triglycerides, releasing fatty acids.
A second organ, the liver, produces yet another digestive juice called bile. Bile is stored between meals in the gallbladder.
At mealtime, it is squeed out of the gallbladder, throught he bile ducts, and into the intestine to mix with the fat in food.
Bile dissolves fat into the watery contents of the intestine, much like detergents that dissolve grease from a frying pan. After fat is dissolved, it is digested by enzymes from the pancreas and the lining of the intestine.

15. The gastrointestinal tract is a long, muscular tube
The gastrointestinal tract is a long, muscular tube. If you were to stretch it out to its full length, it would be about thirty feet long. The tube is so specialized that it actually has its own nervous system.

16. Isotonix Advantage #3
Carbon dioxide created by the effervescent reaction reduces the thickness and viscosity of the mucus layer of the intestinal tract, promoting absorption into the cells.
Pharm. Res. 1998, vol 15, pp “Mechanistic studies on effervescent-induced permeability enhancement.”

17. FOLDS IN THE SMALL INTESTINE Villi Mucosa Sub - Mucosa
Most digested molecules of food, as well as water and minerals, are absorbed through the small intestine. The small intestine contains many folds that are covererd with tiny fingerlike projections called villi.
In turn, the villi are covered with microscopic projections called microvilli. These structures create a vast surface area through which nutrients can be absorbed. The surface area is approximately the size of a tennis court.
Specialized cells allow nutrients to cross the membrane into the blood, where they are transported to other parts of the body for storage or further chemical change.
Sub -
Mucosa 5

19. The Cell Membrane

20. Cell Membrane Barriers
Lipid bi-layers are impermeable to most essential molecules and ions.
Oxygen, Water, & Carbon Dioxide can freely pass.
Many nutrients cannot freely pass.

21. Movement through Differences in Concentration
Diffusion is moving molecules from a region of higher concentration to a region of lower concentration.

22. Simple Diffusion

23. Higher concentrations move nutrients into cells
Membrane (lipid bi-layer)

24. Isotonix Advantage #4
Favorable concentration gradient for absorption of some nutrients. Example: Calcium/Magnesium
Nutrients are delivered to the small intestine in a concentrated solution.

25. Facilitated Diffusion
Takes place through channels embedded in the membrane.
Example: Fructose moved into cell by facilitated diffusion.

26. Active Transport
Pumping of molecules or ions through a membrane into a region of higher concentration. It requires a trans-membrane protein and energy input.
Example: Amino Acids, ATP, Sodium, Potassium moved into cell by Active Transport.

28. SMALL INTESTINE
Absorption of nutrients occurs throughout entire length of small intestine (90 – 95% efficient)
Calcium absorption occurs as late as ileo-cecal juncture (appendix)  transport into cells via calcium channels
Under neutral pH, intrinsic factor binds to vitamin B-12 to form a complex

29. SMALL INTESTINE
Intrinsic factor/vitamin B-12 complex binds to receptors (specialized docking structures) on intestinal membranes
Last 1/3rd of small intestine is main absorptive site

30. COLON
Most nutrients will have been absorbed by the time they reach the large intestine —an additional 3-5% possible.
Major functions of colon:
Site of bacterial cell growth and residence
An estimated species or strains of bacteria continue digestion of waste products
Regulates water in body
Some vitamins produced in low amounts by bacteria and absorbed
Bacteria in the colon play a significant role in our defense system from outside threats. In fact, the largest part of our immune system resides in the lining of the digestive tract.

31. COLON
Bacteria adjust the pH of the colon into the acidic range – prevents overgrowth of harmful bacteria.
Continued growth of friendly bacteria help maintain healthy balance.

32. What does “isotonic” mean?
ISO = SAME
TONIC = Tension or Pressure
An Isotonic Cell is a Happy Cell.

33. Cells act like little bags of salty water…..
The movement of water in and out of a cell is called “Osmosis.”
Cells want the same concentration of dissolved particles (salt) on both sides of the membrane.

35. HYPOTONIC (WATER GOING IN) HYPERTONIC (WATER GOING OUT)
ISOTONIC
(NORMAL)

36. Isotonix Advantage #5
Isotonic solutions are the optimal delivery system.
Isotonix products work with – not against – normal body physiology.

37. Composition of Isotonix Delivery System
Active ingredients: vitamins, minerals, oligomeric proanthocyanidins (OPC), flavonoids, coenzyme Q10, etc.
Base ingredients: fructose, glucose, citric acid, malic acid, calcium sulfate, maltodextrin, silica, potassium bicarbonate

38. Composition of Isotonix Delivery System
Glucose and fructose:
Sweet-tasting simple sugars
Add significant osmotic pressure
Caloric value (~ 8 cal/serving)
Fructose: principle sugar in formula scores 20 out of 100 on glycemic index (i.e., relatively low value)

39. Composition of Isotonix Delivery System
Citric acid and malic acid:
Adjust pH to acid range (about 3.5 – 5.0)
Adjust taste to “citrus” flavor
Minor osmotic pressure adjustment

40. Composition of Isotonix Delivery System
Calcium sulfate:
Desiccant (takes up moisture in powder) / free-flow agent
Minor osmotic pressure adjustment
Minor nutritive value

41. Composition of Isotonix Delivery System
Maltodextrin:
Glucose polymer has minor caloric value
Powder — bulking agent
Prevents citric acid from reacting with potassium bicarbonate and water
Thickening and suspending agent in solution

42. Composition of Isotonix Delivery System
Potassium bicarbonate:
Potassium and bicarbonate ions have significant osmotic pressure
Helps adjust and buffer pH
Release of CO2 stirs liquid

43. Isotonix Advantage #6 Isotonix offers an alternative to pills.
Survey shows 40% of American adults have experienced difficulty swallowing pills
The survey of 679 adults (513 ages 18-64, 166 age 65 and older) was funded by the US affiliate of Germany's Schwarz Pharma.

44. Isotonix Advantage #7
People with stomach and esophageal challenges benefit from Isotonix delivery.
Acid reflux sufferers
Bariatric patients
Hiatal Hernia (stomach protrudes up through diaphragm)
People who have trouble swallowing
Elderly (reduced acid production)

45. Conclusion
Isotonix® product line offers customers a wide selection of nutrient combinations with the advantages of isotonic delivery.
Isotonix® is the logical choice of those seeking fast and optimal nutrient uptake.