1. β-Actin overexpression in hippocampal neurons with ZBP1 knockdown is sufficient for partial phenotype rescue.
β-Actin overexpression in hippocampal neurons with ZBP1 knockdown is sufficient for partial phenotype rescue. A, Micrographs of hippocampal neurons transfected on DIV7 with pEGFP-C1 together with pSuper vector as a control, ZBP1sh#1, or ZBP1sh#1 together with EGFP-ZBP1*1 or EGFP-β-actin. Expression proceeded for 3 d. Neuronal morphology was visualized by staining for cotransfected β-gal. B–F, Mean number of primary dendrites (B), mean number of dendritic tips (C), dendritic branching index (D), mean total dendritic length (E), and Sholl analysis of transfected neurons (F). ***p < , **p < , Kruskal–Wallis test followed by Mann–Whitney post hoc test with Bonferroni's adjustment; ns, not significant. Scale bar, 50 μm. G, Phenotype rescue attempted with other possible ZBP1 targets and unrelated cDNAs. The figure shows representative images of neurons transfected with control plasmid, ZBP1sh#1, ZBP1sh#1/EGFP-ZBP1*1, ZBP1sh#1/c-Myc, ZBP1sh#1/GW1-NR1, ZBP1sh#1/GFP-CaMKIIα, and ZBP1sh#1/MAP2. H, Mean number of dendritic tips of transfected neurons. ***p < , *p < 0.008, Kruskal–Wallis test followed by Mann–Whitney post hoc test with Bonferroni's adjustment; ns, not significant. Scale bar, 50 μm. Error bars indicate SEM.
Malgorzata Perycz et al. J. Neurosci. 2011;31:
©2011 by Society for Neuroscience