1. Increased autoantibodies in Bcl6FC mice.
Increased autoantibodies in Bcl6FC mice. Serum from naive WT and Bcl6FC mice was used to analyze for anti-dsDNA Abs by ELISA and autoantigen microarray. (A and B) Serum from 20-wk-old mice was tested for (A) anti-dsDNA IgG and (B) anti-dsDNA IgA. n = 6 mice; experiment was repeated at least two times. (C) Serum from 5-wk-old mice was tested for anti-autoantigen IgG by microarray. Thirty-eight autoantigens out of 118 autoantigens tested showed a significant difference in IgG reactivity between WT and Bcl6FC, and all 38 showed a higher score for the Bcl6FC sera. All graphs show selected anti-autoantigen IgG scores for anticollagen V, anti-Hmgb1, anti-DNA polymerase β, and anti-Histone H2B taken from the autoantigen array data. n = 4 mice. The p values were calculated by t test, in which **p < 0.01.
Markus M. Xie et al. ImmunoHorizons 2019;3:
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