1. miR-206 regulates the expression of BDNF via three seed matches on its 3′-UTR.
miR-206 regulates the expression of BDNF via three seed matches on its 3′-UTR. A, B, Nanostring verification of BDNF (A) and its receptor, NTRK2, expression (B). C, Target scan identified 3 putative binding targets of miR-206 on the 3′-UTR of BDNF. D, Luciferase activity measured in HEK293T cells cotransfected with either miR-206 mimic, miR-9 mimic, or scrambled mimic, and a luciferase reporter plasmid carrying the 3′-UTR of BDNF. Renilla luciferase activity was normalized by firefly luciferase expression levels and is presented as percentage of activity achieved by the 3′-UTR of BDNF in the presence of either mimic (miR-206, miR-9, scrambled). E, Luciferase activity measured in HEK293T cells with miR-206 or scrambled mimic and a luciferase reporter plasmid with mutations at site 1 (214–220), site 2 (392–398), site 3 (1294–1300), combinations with these sites, or all sites mutated. **p < 0.01, *p < 0.05, #p = 0.05–0.1 (trend significance).
Jenica D. Tapocik et al. J. Neurosci. 2014;34:
©2014 by Society for Neuroscience