1. Molecular characterization of the key motifs of AnMac1.
Molecular characterization of the key motifs of AnMac1. (A) Schematic view of the AnMac1 protein showing Cys, RGHR, and GRP residues in the N-terminal Cu fist structure as well as C-terminal Cys-rich motifs REP-I and REP-II. Black and green ovals indicate the positions of Cys and His residues, respectively. (B) Characterization of Cys, RGHR, and GRP mutants of A. nidulans grown on MMPDR media supplemented with 1 μM Cu at 37°C for 40 h. (C) Phenotypic characterization of the REP-I and REP-II A. nidulans mutant strains after growth on MMPDR media supplemented with 1 μM Cu at 37°C for 40 h. (D) A. fumigatus REP-I and REP-II mutant strains grown at 37°C for 40 h on 1 µM Cu-supplemented MM media. For panels B, C, and D, closeup views of the center of individual colonies were taken with a stereo microscope; the bars in those panels represent 100 μm.
Zhendong Cai et al. mSphere 2019; doi: /mSphere