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Published byNoëlle Lecompte Modified over 5 years ago
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3DMorph workflow. 3DMorph workflow. The user selects either Interactive or Automatic mode. Interactive mode must be used first to generate a parameters file. A, The user then selects the file to analyze, and specifies x, y, and z scale, number of channels, and the channel of interest. Both .tiff and .lsm files are supported. The original image (B) is loaded and 3D connected components (C) are automatically detected. D, Large cells can be selected for segmentation and small objects can be removed. E, After skeletonization and measurements of remaining cells, 3DMorph saves selected images and generates an Excel results file. Gray text indicates automatic steps. Elisa M. York et al. eNeuro 2018;5:ENEURO ©2018 by Society for Neuroscience
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