1. Estradiol but not progesterone increases overall excitability and burst events in AVPV kisspeptin neurons.
Estradiol but not progesterone increases overall excitability and burst events in AVPV kisspeptin neurons. A, Representative extracellular recordings of OVX, OVX+E, OVX+E+P, and OVX+E+V with AMPA, NMDA, and GABAA receptors antagonized. Gray boxes indicate bursts. B, C, Mean ± SEM firing frequency (B) and number of burst events (C) of OVX (black circles), OVX+E (solid red circles), OVX+E+V (open red circles), and OVX+E+P (blue). D, Percentage of cells that fire DIB or tonic patterns on OVX, OVX+E, and OVX+E+P groups. E, Representative firing properties of tonic (top) and DIB (bottom; except OVX) cells in OVX, OVX+E, and OVX+E+P groups. F–H, Mean ± SEM of action potential parameters in OVX (white), OVX+E (hatched), and OVX+E+P (black) groups: latency to first spike (F), action potential FWHM (G), and AHP potential (amplitude; H). *p < 0.05 calculated by one-way ANOVA/Bonferroni test or Kruskal–Wallis or Dunn’s test, as dictated by data distribution.
Luhong Wang et al. eneuro 2016;3:ENEURO
©2016 by Society for Neuroscience