1. Phenotype of S. pombe cells in the presence of B21P2 or LMB
Phenotype of S. pombe cells in the presence of B21P2 or LMB. A total of 100 cells of strain PN5352 were examined for the major phenotypes of asymmetric nucleus (AN), extended spindle (ES) and asymmetric septum (AS) in the presence of B21P2 or LMB. Examples of S. pombe cells illustrating these defects are shown.
Phenotype of S. pombe cells in the presence of B21P2 or LMB. A total of 100 cells of strain PN5352 were examined for the major phenotypes of asymmetric nucleus (AN), extended spindle (ES) and asymmetric septum (AS) in the presence of B21P2 or LMB. Examples of S. pombe cells illustrating these defects are shown. (A,B) Cells in absence of drug (AN, n=0/100 cells; ES, n=0/100 cells; AS, n=0/100 cells). (C,D) Cells+B21P2 (1:100 dilution; AN, n=43/100 cells; ES, n=41/100 cells; AS, n=29/39 septated cells). (E,F) Cells+LMB (50 ng/ml; AN, n=30/100 cells; ES, n= 45/100 cells; AS, n=18/34 septated cells). Examples of asymmetric nucleus (green arrows), extended spindle (yellow arrows) and asymmetric septation (white arrows) are highlighted. Many cells show multiple defects. PN5352 cells were incubated in B21P2 or LMB for 2 h. Scale bars: 5 µm.
Richard A. Lewis et al. J Cell Sci 2017;130:
© Published by The Company of Biologists Ltd