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Recruitment of epiplakin to keratin filaments and aggregates after application of various types of stress. Recruitment of epiplakin to keratin filaments and aggregates after application of various types of stress. Primary mouse keratinocytes isolated from wild-type (+/+) or epiplakin-deficient (–/–) mice were treated with orthovanadate (OV) for 2 minutes and 10 minutes, or were exposed to 150 mM urea or UV (400 J/m2) prior to immunolabeling using antibodies as indicated. Merged images of wild-type cells show colocalization of epiplakin and keratin. Scale bar: 10 μm. Daniel Spazierer et al. J Cell Sci 2008;121: © The Company of Biologists Limited 2008
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