1. IFN-γ prevents SAG-induced inhibition of adipocyte differentiation.
IFN-γ prevents SAG-induced inhibition of adipocyte differentiation. A: 3T3-L1 cells were induced to differentiate into adipocytes in the presence of 20 nmol/L SAG and/or 50 ng/mL IFN-γ or were left untreated (Con) for 10 days. OrO staining was performed 10 days after induction. B: Adipocytes were counted under a bright-field microscope and presented as the percentage of the total number of methylene blue–stained nuclei. C: mRNA levels of Hh target genes (Gli1, Hhip, and Ptch1) were measured by quantitative RT-PCR analysis and normalized to Rplp0. D: Gli1 Western blot of whole-cell lysates. Heat shock protein 70 was used as the loading control. E: mRNA levels of marker genes of adipocyte differentiation (aP2/Fabp4, Adipoq, Cebpα, and Pparγ) were measured by quantitative RT-PCR analysis. Data represent means ± SEM of triplicate samples. *P < 0.05; **P < 0.01; ***P < 0.001; NS, not significant. (A high-quality color representation of this figure is available in the online issue.)‏
Jelena Todoric et al. Diabetes 2011;60:
©2011 by American Diabetes Association