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Expression of Sézary Biomarkers in the Blood of Patients with Erythrodermic Mycosis Fungoides  Charlotte Hurabielle, Laurence Michel, Caroline Ram-Wolff,

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Presentation on theme: "Expression of Sézary Biomarkers in the Blood of Patients with Erythrodermic Mycosis Fungoides  Charlotte Hurabielle, Laurence Michel, Caroline Ram-Wolff,"— Presentation transcript:

1 Expression of Sézary Biomarkers in the Blood of Patients with Erythrodermic Mycosis Fungoides 
Charlotte Hurabielle, Laurence Michel, Caroline Ram-Wolff, Maxime Battistella, Francette Jean-Louis, Marie Beylot-Barry, Michel d’Incan, Armand Bensussan, Martine Bagot  Journal of Investigative Dermatology  Volume 136, Issue 1, Pages (January 2016) DOI: /JID Copyright © 2015 The Authors Terms and Conditions

2 Figure 1 Flow cytometry analysis and blood biomarker qPCR values in EMF patients. (a) Flow cytometry analysis of CD3+CD4+KIR3DL2+ cells in one representative patient with EMF at stage B1. Percentage of CD3+CD4+ T cells was ascertained among fresh peripheral blood mononuclear cell gate (left), and then KIR3DL2 expression was evaluated on the CD3+CD4+ T cells by immunofluorescence staining with a monoclonal phycoerythrin–conjugated KIR3DL2 antibody (Miltenyi) (right). The blue line (empty histogram) indicates staining with a control isotype monoclonal irrelevant antibody. (b) Individual values of each blood biomarker in B0 and B1 patients. All samples were run in duplicate. The single data points in (b) represent the mean value of the duplicate for each blood biomarker for each individual patient. The bars represent median values, and the error bars are the interquartile range. Mann-Whitney test: *P < EMF, erythrodermic mycosis fungoides. Journal of Investigative Dermatology  , DOI: ( /JID ) Copyright © 2015 The Authors Terms and Conditions

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