1. Responsiveness of syzygial preparations.
Responsiveness of syzygial preparations. The responsiveness was categorised as A, B or C, as explained in the Materials and methods section. Note that, although, in the bar charts, the results are proportionated to percentages, statistical tests were applied to actual numbers of preparations showing each type of response. (A) Effect of longitudinal position syzygial preparations in the arm (‘1’ being nearest the base; tests on 20 preparations from each position; preparations obtained from five arms of each of four animals); stimulatory agent 100 mmol l−1 K+. Ordinal logistic regression analysis indicated that the decline in responsiveness from the base of the arm is significant (P<0.001) (see Appendix). (B) Effect of pretreatment for 30–60 min with 1 mmol l−1 procaine; stimulatory agent 100 mmol l−1 K+. In B, C and D, the numbers of preparations in treated and control groups are given in parentheses, and probabilities generated by Mann–Whitney tests comparing treated and control batches are shown above the treatment bars. (C) Effects of pretreatment with chlorpromazine (1 mmol l−1 for 30 min) and the more-specific glutamate receptor antagonists ketamine (1 mmol l−1 for 30 min), kynurenate (5 mmol l−1 for 30 min) and CNQX (1 mmol l−1 for 10 min); stimulatory agent 1 mmol l−1 l-glutamate for chlorpromazine and ketamine and 5 mmol l−1 l-glutamate for kynurenate and CNQX. Relevant control results are placed to the right of those for each treatment. (D) Effect of pretreatment for 3 min with 1 mmol l−1 acetylcholine; stimulatory agent 2 mmol l−1 l-glutamate.
I. C. Wilkie et al. J Exp Biol 2010;213:
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