1. FISH with pooled large-insert clones representing chromosome 17 early-replicating (green) and chromosome 5 late-replicating (red) loci to (A) human (schematics of human chromosomes 5 and 17, together with the mapping position of large-insert clones, are shown alongside) and (B) Concolor gibbon metaphase chromosomes. 3D maximum intensity projections of nuclei from (C) human, (D) gorilla, (E) Concolor gibbon lymphoblastoids and (F) Mel Juso tumor cells hybridized with these probe pools.
FISH with pooled large-insert clones representing chromosome 17 early-replicating (green) and chromosome 5 late-replicating (red) loci to (A) human (schematics of human chromosomes 5 and 17, together with the mapping position of large-insert clones, are shown alongside) and (B) Concolor gibbon metaphase chromosomes. 3D maximum intensity projections of nuclei from (C) human, (D) gorilla, (E) Concolor gibbon lymphoblastoids and (F) Mel Juso tumor cells hybridized with these probe pools. In C-F, chromosome 17 early-replicating clones are labeled green, chromosome 5 late-replicating clones red, chromosome 5 and 17 territories blue and DAPI counterstain gray, except for F, in which the wild-type CT17 is shown in blue and the CT der(17) in red. Scale bar: 5 μm. (G-L) Quantitative evaluation of radial probe distributions in the interphase nucleus or the respective chromosome territory, using 3D-RRD or eADS software (n=number of nuclei).
Florian Grasser et al. J Cell Sci 2008;121:
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