1. CD25 surface expression and TCR signal strength predict T helper differentiation and memory potential of early effector T cells in vivo.
CD25 surface expression and TCR signal strength predict T helper differentiation and memory potential of early effector T cells in vivo. (A) SMARTA T cells (Thy1.1+) were adoptively transferred (3 × 104) into B6 hosts (Thy1.2+), followed by LCMV infection. Representative flow histograms indicate the CD25 surface expression by SMARTA T cells at days 0 to 5 (d0 to d5) after LCMV infection. (B) The representative flow histogram shows expression of CD25 by SMARTA cells at day 3 post-infection and expression of Tim-3 and Ly6C by CD25hi and CD25lo subsets. (C) The bar graphs indicate the MFI of CXCR5, T-bet, TCF-1, and Bcl-6 in CD25hi and CD25lo SMARTA CD4+ T cells in the spleen at day 3 post-infection. (D) The bar graph indicates ΔpZAP-70 MFI of CD25hi (“High”) and CD25lo (“Low”) SMARTA CD4+ T cells in the spleen at days 3 and 5 post-infection. Error bars indicate the SEM, and statistical significance was determined by Student’s t test (n = 4 mice per group, representative of four independent experiments). *P < 0.05, **P < 0.01, ***P < 0.001, ****P <
Jeremy P. Snook et al. Sci. Immunol. 2018;3:eaas9103
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