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Figure 2 Immunoblotting of erythrocyte membranes, lymphoblastoid cells, and co-immunoprecipitants Immunoblotting of erythrocyte membranes, lymphoblastoid.

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Presentation on theme: "Figure 2 Immunoblotting of erythrocyte membranes, lymphoblastoid cells, and co-immunoprecipitants Immunoblotting of erythrocyte membranes, lymphoblastoid."— Presentation transcript:

1 Figure 2 Immunoblotting of erythrocyte membranes, lymphoblastoid cells, and co-immunoprecipitants
Immunoblotting of erythrocyte membranes, lymphoblastoid cells, and co-immunoprecipitants (A and C) Chorein immunoblotting results revealed reduced chorein immunoreactivity in the erythrocyte membranes of patients with MLS, which are equivalent to the heterozygous mutant carriers of ChAc (ChAc_hetero). (B and C) XK immunoblotting results revealed normal XK immunoreactivity in the erythrocyte membranes of ChAc and ChAc_hetero. (A–C) Each imaging was performed by underexposed condition for semiquantitative analysis. (D) The histograms show the chorein relative density ratio in patients with MLS and ChAc and ChAc_hetero. Each band density was normalized by protein density staining with MemCode reversible protein stain. Error bars represent the SD (each n = 6); 2-sample Student t tests were performed accordingly on different sets of data. **p < 0.01; ***p < d shows the effect size (Cohen's d). (E) The histograms show the XK protein relative density ratio in patients with MLS and ChAc and ChAc_hetero. Each band density was normalized by protein density staining with memcode reversible protein stain. (F) Chorein immunoblotting results revealed equivalent chorein immunoreactivity in the lymphoblastoid cells of MLS_1 and the control (MLS_1_lympho and Control_lympho, respectively). The XK immunoblotting of lymphoblastoid cells shown in the lower panel of figure 2F reveals a lack of XK immunoreactivity. The Control_EM and MLS_EM lanes show the erythrocyte membrane for control and MLS, respectively. (G) Co-immunoprecipitation (IP) assay using K562 cells was performed with anti-XK antibody. Immunoblot analyses used anti-chorein (upper panel) and anti-XK antibodies (lower panel). (H) Co-IP and reverse co-IP assays using human embryonic kidney 293 (HEK293) cells stably overexpressing Myc-DDK–tagged chorein confirmed an interaction between XK protein and chorein. Immunoblot analyses used anti-XK protein and anti-chorein antibodies. Yuka Urata et al. Neurol Genet 2019;5:e328 © The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the 2019 American Academy of Neurology.

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