1. Autophagic vacuoles were frequently detected in the perinuclear area close to the Golgi apparatus.
Autophagic vacuoles were frequently detected in the perinuclear area close to the Golgi apparatus. HeLa cells were starved for 2 hours to induce autophagy and prepared for immunogold labelling of LC3 (A) or Lamp1 (B) followed by a secondary antibody coupled to 5 nm gold (arrowheads). Autophagic vacuoles (AV) were frequently observed to accumulate close to the nucleus (Nu) and the Golgi stack (G), where endo/lysosomal vacuoles (LE) were also present. Some autophagic vacuoles were observed to fuse with the Lamp1-positive endo/lysosomal vacuoles (B).
Stefanie Jäger et al. J Cell Sci 2004;117:
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