1. Detection of GPR55 ligand in the small intestine.
Detection of GPR55 ligand in the small intestine. (A) Abundance of the indicated forms of LPI in spleen (n = 5) and small intestine (n = 5) tissue determined by LC-MS/MS. (B and C) Bioassay measurement of relative GPR55 ligand activity in small intestine, colon, and spleen extract (B) and in small intestinal epithelial cell (SI-EC) culture supernatants (C). WEHI231 cells transduced with GPR55 were tested for their response to the tissue extracts in a Transwell migration assay. Extract at the indicated dilution was mixed with CXCL12 (100 ng/ml). Each experiment was performed in duplicate and repeated three times. (D) Histograms showing Thy1.1 reporter expression on intestinal and spleen DCs in mice that had been reconstituted with empty vector– or GPR55-transduced BM. (E to G) Summary data from mice of the type in (D), shown as the ratio of Thy1.1+/Thy1.1− cells of the indicated types within the intestine (E and F) and spleen (G). n = 4 in each group. **P < 0.01, *P < 0.05, n.s. P > 0.05 by Student’s t test.
Hayakazu Sumida et al. Sci. Immunol. 2017;2:eaao1135
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