1. Expedited loss of MacroH2A
Expedited loss of MacroH2A.1 variants and increased levels of H3me2K4 in APLF-depleted fibroblasts enhances reprogramming.
Expedited loss of MacroH2A.1 variants and increased levels of H3me2K4 in APLF-depleted fibroblasts enhances reprogramming. (A) qRT-PCR analysis for the gene expression of Macroh2A.1 in MEFs and E14 ESCs. (B) ChIP analysis was performed with control (MEF-plko.1-OSKM) and Aplf-kd (MEF-Aplf shRNA-OSKM) cells at day 9 of reprogramming. The plots represent the level of MacroH2A.1 variant at different promoters. IgG was used as the negative control. (C) The same sets of cells as described in B were analyzed for the presence of different histone modifications. (D,E) The same sets of cells in C were analyzed for enrichment of H3me2K4 at different loci by performing ChIP analyses. (F) Proposed model for the regulation of reprogramming by APLF. Error bars are s.e.m., n=3, *P<0.05, **P<0.01 (Student's t-test).
Khaja Mohieddin Syed et al. J Cell Sci 2016;129:
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