1. Altered micro-ribonucleic acid expression profiles of extracellular microvesicles in the seminal plasma of patients with oligoasthenozoospermia 
Masood Abu-Halima, Ph.D., Nicole Ludwig, Ph.D., Martin Hart, Ph.D., Petra Leidinger, Ph.D., Christina Backes, Ph.D., Andreas Keller, Ph.D., Mohamad Hammadeh, Ph.D., Eckart Meese, Ph.D. 
Fertility and Sterility 
Volume 106, Issue 5, Pages e3 (October 2016)
DOI: /j.fertnstert
Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

2. Supplemental Figure 1
Examples of RNA content by capillary electrophoresis by using an Agilent 2100 Bioanalyzer and an RNA Nano chip. The RNAs isolated from extracellular microvesicles collected from seminal plasma show very low integrity, with RNA integrity number <2.8.
Fertility and Sterility  , e3DOI: ( /j.fertnstert )
Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

3. Supplemental Figure 2
Western blot analysis of the exosomal marker proteins CD63 (approximately 53 kDa), CD81 (approximately 26 kDa), CD9 (approximately 28 kDa), and HSP70 (approximately 70 kDa) for samples of oligoasthenozoospermic and normozoospermic men. Microvesicles isolated with ultracentrifugation protocols were separated on a 10% standard SDS-PAGE. Standard Western blot procedures with anti-CD63, anti-CD81, anti-CD9, and anti-HSP70 antibodies were used to detect exosomal protein markers.
Fertility and Sterility  , e3DOI: ( /j.fertnstert )
Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

4. Supplemental Figure 3
Heatmap based on the differential expression of the 36 altered miRNAs in oligoasthenozoospermic and normozoospermic men. Columns represent seminal plasma microvesicle samples, and rows represent miRNAs. Dark blue indicates high expression, and dark red low expression.
Fertility and Sterility  , e3DOI: ( /j.fertnstert )
Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions