1. A B D C E F Supplementary Figure S4. Epping et al. IP: flag input IgG
150
100 75 50
flag- TSPYL2
SIN3A 37 25 20
FL FL
FL FL TSPYL2
Interaction of TSPYL2 domains with SIN3A A B
Gal4-luciferase
0.1
0.2
0.3
0.4
0.5
0.6
Gal4
Gal4-
T2 (FL)
T2 (1)
T2 (2)
T2 (3)
Relative luciferase activity IP
REST (1-323) REST ( )
flag-REST
(1-323)
TSPYL2
CoREST
SIN3A
( )
IgG flag
input D C
1-1097
1-323 E IP
REST ( ) REST ( )
IgG flag
input
flag-REST
( )
( )
TSPYL2
SMAD4
TSPYL2
actin
GFP
TSPYL2#1
TSPYL2#2
SMAD4#1
shRNA F