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Overexpression of GFP-fused PH domain of Gcs1p did not affect distribution of mitochondrial or ER markers or actin. Overexpression of GFP-fused PH domain of Gcs1p did not affect distribution of mitochondrial or ER markers or actin. Immunofluorescence staining of gcs1 cells overexpressing GFP-fused PH domain and reacted with purified anti-Gcs1p antibody (c, g and k), anti-porin antibody (d), anti-Kar2 antibody (h) or Alexa FluorTM 594-phalloidin (l), or stained with H33258 (b, f and j). Chun-Fang Huang et al. J Cell Sci 2002;115: © The Company of Biologists Limited 2002
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