1. Diaphanous is required presynaptically for normal synaptic growth.
Diaphanous is required presynaptically for normal synaptic growth. A–E , Immunofluorescent images of third-instar NMJs on muscles 6/7 ( A , B ) or muscle 4 ( C–E ) from wild type ( A , C ), dia 2/2 mutants ( B , D ), or presynaptic dia rescue animals ( E ). The dia 2/2 mutant phenotypes are rescued by presynaptic expression of UAS-Dia Act in the dia 2 /2 background using a neuronal (elaV-GAL4) driver ( E ). Synapses were stained using the anti-HRP antibody. Scale bar, 20 μm. F , Quantification of number of boutons per NMJ normalized to wild-type synapses. Error bars represent SEM. Asterisks indicate statistically significant differences compared with wild type.
Catherine Pawson et al. J. Neurosci. 2008;28:
©2008 by Society for Neuroscience