1. Differential Effects of Cytokines and Immunosuppressive Drugs on CD40, B7–1, and B7–2 Expression on Purified Epidermal Langerhans Cells 
Claudio Guedes Salgado, Koichiro Nakamura, Makoto Sugaya, Yayoi Tada, Akihiko Asahina, Kunihiko Tamaki 
Journal of Investigative Dermatology 
Volume 113, Issue 6, Pages (December 1999)
DOI: /j x
Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

2. Figure 1
Panning technique yields highly purified Langerhans cells (with around 95% purification). The same field of freshly isolated epidermal Langerhans cells, stained with FITC-Iad MoAb observed under a fluorescence microscope (a) and light microscope (b). Highly purified Langerhans cells were analyzed by FACS (c). The solid line represents staining with FITC-Iad MoAb, and the dotted line represents staining with FITC-IgG. Electron micrographs of purified Langerhans cells are shown (d, e). Freshly isolated Langerhans cells contain Birbeck granules. Scale bar: 0.5 μm.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

3. Figure 2
Cytokine and cytokine receptor mRNA profiles of freshly isolated Langerhans cells. mRNA was isolated from fLC and cDNA was amplified using the primers described in Materials and Methods. PCR products are shown in the figure. The expected size of bands were as follows: M-CSFR, 438 bp; GM-CSFR, 455 bp; TNFRII, 636 bp; IL-1β, 563 bp; and TNF-α, 692 bp. G3PDH (983 bp) was used as a control.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions