1. Dissection and clearing of brain subregions.
Dissection and clearing of brain subregions. As it may not be necessary to clear the entire brain, it is more efficient to dissect the region of interest before clearing and imaging. Examples of dissected hippocampus and olfactory bulbs before and after clearing and subsequently stained with propidium iodide are shown. Electrophoretic tissue clearing was performed for 3 d (2 d at 37°C and 1 d at 55°C). Imaging of the cleared tissue was performed with a Zeiss 710 confocal microscope and 10× objective.
Jonathan R. Epp et al. eneuro 2015;2:ENEURO
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