1. Diaphanous is present presynaptically and postsynaptically.
Diaphanous is present presynaptically and postsynaptically. A , Diagram of the Diaphanous locus with protein coding sequences (CDS) and untranslated sequences (UTR) indicated. Small deletions associated with the dia 2 and dia 9 mutants are shown (Castrillon and Wasserman, 1994). B , Diagram of Dia protein including the GBD, the coiled-coil region (CC), and the formin homology domains 1 and 2 (FH1 and FH2, respectively). The percentage amino acid sequence identity between Drosophila Dia protein and the mouse Dia2 protein (mDia2) is indicated. C , Immunofluorescent image of a third-instar NMJ from wild-type (left two panels) and a dia 2 larva (right two panels) stained with anti-Dia (top) and anti-HRP (bottom). The arrow indicates Dia staining of putative endosomes postsynaptically. Note that staining is nearly absent at the dia 2 mutant NMJ. D , Three-dimensional imaging demonstrates colocalization of Dia protein (left panel) and the Futsch protein (center panel) within a single presynaptic bouton. The right panel represents a merged image of the staining of the Dia and Futsch proteins. The arrow indicates Dia protein closely associated with presynaptic Futsch bound microtubules. The insets above represent the z-axis cut as a horizontal line through the indicated Dia puncta (arrow). The insets to the left represent the z-axis cut as a vertical line through the same puncta.
Catherine Pawson et al. J. Neurosci. 2008;28:
©2008 by Society for Neuroscience