1. ITS rRNA gene locus.
ITS rRNA gene locus. Schematic of the eukaryotic ribosomal gene cluster. The SILVA database contains sequences of the 18S gene, while the UNITE database contains sequences from the ITS1-5.8S-ITS2-25S rRNA gene cluster (not to scale). For development of our custom primers, we created a merged SILVA and UNITE database to simulate the 18S-ITS1-5.8S region. A 250-bp region at the 3′ end of the 18S gene was individually isolated when designing the forward primers.
Mykhaylo Usyk et al. mSphere 2017; doi: /mSphere