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Step 1 Step 2 Step 3 Step 4 FOR Ligation Chart

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Presentation on theme: "Step 1 Step 2 Step 3 Step 4 FOR Ligation Chart"— Presentation transcript:

1 Step 1 Step 2 Step 3 Step 4 FOR Ligation Chart
J3701 (Alternate Method) C I13504 F2620+(C0261+I13504) MAKES PART J37015! J37016 Predator cell transfer function test (poly-cistronic) J I13504 MAKES PART J37016 !! J37019 Intermediate Part C B0034 R (C0062+B0034) Add Aiia part for final construct (for monocistronic mRNA) J37020 Predator Transfer Function (mono-cist) Wait for part I13033 to grow! I I13504 J _______ MAKES PART J37020 !! J37020 (Alternate Method) J I13033 Add I13504 to get part J37020 (to test previous ligation worked ) Add Aiia part for final construct

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