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Differential alterations of metabolites associated with synthesis of lipopolysaccharide (A) and peptidoglycan (B) in strains PAK and PAKpmrB6 in response to polymyxin B treatment (4 mg/liter) at 1 and 4 h. Differential alterations of metabolites associated with synthesis of lipopolysaccharide (A) and peptidoglycan (B) in strains PAK and PAKpmrB6 in response to polymyxin B treatment (4 mg/liter) at 1 and 4 h. Blue boxes in the flow charts indicate that the metabolites were differentially changed, while gray boxes indicate that the metabolites were not detected by either the HILIC or RPLC method. Statistical significance (Student’s t test) is indicated by asterisks as follows: ∗, P < 0.05; ∗∗, P < 0.01; ∗∗∗, P < Mei-Ling Han et al. mSystems 2019; doi: /mSystems
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