1. Fig. 3 FcRL1 is passively recruited into B cell immunological synapses upon BCR engagement in primary spleen B cells.
FcRL1 is passively recruited into B cell immunological synapses upon BCR engagement in primary spleen B cells. (A) Representative TIRFM images showing the synaptic accumulation of BCR or FcRL1 in primary B cells stimulated with 30 nM F(ab′)2 goat anti-mouse MHC I, 30 nM F(ab′)2 rabbit anti-HA, 30 nM F(ab′)2 goat anti-mouse IgM, or 30 nM F(ab′)2 goat anti-mouse IgM + 30 nM F(ab′)2 rabbit anti-HA. Scale bars, 1.5 μm. (B) Colocalization analyses of BCR and FcRL1 microclusters within B cell immunological synapses (n = 33 to 36 cells). Experiments were repeated three times, and data from a representative experiment are shown. Bar represents means ± SEM. Two-tailed Student’s t tests were used for the statistical comparisons. (C and D) Statistical quantification of accumulated FcRL1 (C) (n = 31 to 34 cells) and BCR (D) (n = 31 to 35 cells) within B cell immunological synapses. Experiments were repeated three times, and data from a representative experiment are shown. Bar represents means ± SD. Each symbol represents one cell. Two-tailed Student’s t tests were used for statistical comparisons.
Xingwang Zhao et al. Sci Adv 2019;5:eaaw0315
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