1. Role of TT2 and TT16 in PA Biosynthesis
Role of TT2 and TT16 in PA Biosynthesis.(A) to (C) Roots of 10-day-old transformants expressing ProBAN1:uidA (A), ProBAN1:uidA in a Pro35Sdual:TT2 background (B), and Pro35Sdual:uidA used as a positive control (C).(D) to (I) Pattern of TT2 promoter activity...
Role of TT2 and TT16 in PA Biosynthesis.(A) to (C) Roots of 10-day-old transformants expressing ProBAN1:uidA(A), ProBAN1:uidA in a Pro35Sdual:TT2 background (B), and Pro35Sdual:uidA used as a positive control (C).(D) to (I) Pattern of TT2 promoter activity in developing seeds revealed by the detection of GUS in transformants expressing ProTT2:uidA.(D) to (G) Whole mounts were observed with Nomarski optics in a young fertilized ovule (D) and during the quadrant stage of embryo development (E), the early globular stage (F), and the globular stage (G).(H) Seed section showing a detailed view of the micropyle/chalaza area.(I) Seed section showing a detailed view of GUS activity in endothelial basal cells at the chalaza.(J) to (M) Ectopic expression of TT2 in the tt16-1 background.(J) Phenotype of mature dry seeds.(K) Vanillin assay on a developing seed at the heart stage of embryo development showing the presence of PAs in endothelial cells (white arrowhead) and ectopically in cells of the above-situated integumentary layers (black arrowhead).(L) Seed longitudinal section.(M) Seed transverse section.C, chalaza; CEC, chalazal endosperm cyst; CPT, chalazal proliferating tissue; Ct, cotyledon; EM, embryo; EN, endosperm; H, hypocotyl; ii, inner integument; M, micropyle; PS, pigment strand. Bar in (M) = 35 mm for (A), 25 mm for (B) and (C), 12 mm for (J), 80 μm for (L), 75 μm for (F) and (G), 50 μm for (E) and (M), 30 μm for (D), (H) and (I), and 65 μm for (K).
Isabelle Debeaujon et al. Plant Cell 2003;15:
©2003 by American Society of Plant Biologists