1. Brain and plasma cholesterol levels in Abca1−B/−B mice.
Brain and plasma cholesterol levels in Abca1−B/−B mice. A, Selective loss of ABCA1 in the brains of Abca1−B/−B mice. Tissue lysates from different brain regions, liver, and small intestine from control (+/+) and Abca1−B/−B mice were analyzed by immunoblotting using a polyclonal antibody for ABCA1. GAPDH was used as a loading control. Densitometry analysis of the liver and intestine blot is shown (means ± SEM, n = 4). B, Cholesterol levels in cortex and hippocampus were analyzed by gas-liquid chromatographic mass spectrometry. **p < 0.01 versus control littermates, n = 6 per group. C, Levels of total cholesterol (TC), HDL-cholesterol (HDL-C), phospholipids (PL), and triglycerides (TG) in plasma measured by enzymatic assays. *p < 0.05, versus control littermates, n = 12–20 per group. Ctx, Cortex; Hipp, hippocampus; Cb, cerebellum; BG, basal ganglia.
Joanna M. Karasinska et al. J. Neurosci. 2009;29:
©2009 by Society for Neuroscience