1. Levels of apoptosis in human granulosa cells seem to be comparable after therapy with a gonadotropin-releasing hormone agonist or antagonist 
Franca Giampietro, M.D., Ph.D., Silvia Sancilio, Ph.D., Gian Mario Tiboni, M.D., Rosa Alba Rana, M.D., Roberta Di Pietro, M.D. 
Fertility and Sterility 
Volume 85, Issue 2, Pages (February 2006)
DOI: /j.fertnstert
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

2. FIGURE 1
Results of flow cytometric analysis of granulose cells of one representative experiment (GnRH-agonist-treated women). Early apoptotic cell populations (Annexin V+/PI−) can be discriminated from vital (Annexin V−/PI−) or necrotic cells (Annexin+/PI+) according to their fluorescence emission. Cells in quadrant D4 are apoptotic. Quadrants D1 and D2 represent dead and damaged cells, respectively, and vital cells are situated in quadrant D3.
Giampietro. GnRH agonist/antagonist and apoptosis. Fertil Steril 2006.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

3. FIGURE 2
Phase contrast and fluorescent micrographs showing granulosa cell apoptotic (A) and normal (N) nuclei assayed with the terminal deoxynucleotidyl transferase (TdT)-mediated nick-end labeling (TUNEL) technique. Original magnification, ×40.
Giampietro. GnRH agonist/antagonist and apoptosis. Fertil Steril 2006.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

4. FIGURE 3
Deoxyribonucleic acid histogram of granulose cells (GCs) stained with PI and analyzed with flow cytometry. The inset shows a representative field of GC nuclei counterstained with 6-diamino-2-phenylindole (DAPI) and observed with fluorescent microscopy. No evidence of typical micronuclei was observed.
Giampietro. GnRH agonist/antagonist and apoptosis. Fertil Steril 2006.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

5. FIGURE 4
(A) Electron micrograph showing a typical granulose cell (GC) with a large, round, and eccentric nucleus (Nu) with the presence of dispersed chromatin and distinctly visible nucleoli (white arrows). Note abundant osmiophilic lipid droplets scattered throughout the cytoplasm (black arrows). Well-preserved mitochondria (M) with complex tubular cristae are often located adjacent to electron-dense droplets. Original magnification, ×7,000; ×50,000 in inset. (B) Apoptotic GC with the aspect of peripheral chromatin condensation and margination. Original magnification, ×4,000. (C) A normal GC which is removing by phagocytosis a highly condensed nucleus (black arrow) of an apoptotic cell. Original magnification, ×4,000.
Giampietro. GnRH agonist/antagonist and apoptosis. Fertil Steril 2006.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions