1. The absence of Krp1 does not alter capsule production nor chitooligomers and mannose distribution in cryptococcal cell surface.
The absence of Krp1 does not alter capsule production nor chitooligomers and mannose distribution in cryptococcal cell surface. (A) India ink staining of C. gattii cells grown under capsule-induced conditions. Bars, 10 µm. (B and C) C. gattii cells were grown under capsule-induced conditions supplemented with Congo red (625 µg/ml) and labeled with the anti-GXM monoclonal antibody 18B7 to detect capsule (red) and with WGA (green) (B) to detect chitooligomeric structures. Alternatively, such cells were also labeled with the lectin ConA (green) (C) to detect mannosyl residues. Images were captured by a fluorescence confocal microscope. Micrographs were taken at a magnification of ×630.
Julia Catarina Vieira Reuwsaat et al. mSphere 2018; doi: /mSphere