1. Electroencephalographic coherence and cortical acetylcholine during ketamine-induced unconsciousness 
D. Pal, V.S. Hambrecht-Wiedbusch, B.H. Silverstein, G.A. Mashour 
British Journal of Anaesthesia 
Volume 114, Issue 6, Pages (June 2015)
DOI: /bja/aev095
Copyright © 2015 The Author(s) Terms and Conditions

2. Fig 1
Schematic diagram illustrating the temporal course of experimental intervention and data collection. ACh, acetylcholine; i.p., intraperitoneal; LORR, loss of righting reflex; RORR, return of righting reflex.
British Journal of Anaesthesia  , DOI: ( /bja/aev095)
Copyright © 2015 The Author(s) Terms and Conditions

3. Fig 2
(a) Cresyl violet-stained representative coronal brain section through prefrontal cortex showing the dialysis probe track and the site of microdialysis. Arrow indicates the ventral tip of the dialysis membrane (1 mm long). (b) Coronal brain section drawings from the rat brain atlas of Paxinos and Watson18 to illustrate the location of microdialysis probes (vertical cylinders) within the prefrontal cortex. The numbers within coronal sections show the anterior–posterior location of each coronal section relative to bregma. (c) Representative acetylcholine (ACh) chromatogram showing the signal-to-noise ratio and the retention time for ACh and choline (Ch) peaks. PrL, prelimbic area in the prefrontal cortex.
British Journal of Anaesthesia  , DOI: ( /bja/aev095)
Copyright © 2015 The Author(s) Terms and Conditions

4. Fig 3
Effect of ketamine on coherence and power spectral density (PSD). (a) Representative coherogram showing a significant decrease in coherence during ketamine-induced unconsciousness (Ketamine) and the appearance of high-frequency gamma coherence during emergence and after the return of righting reflex (RORR). (b) Statistical comparisons (repeated measures anova) of changes in coherence between Wake (blue), Ketamine-induced unconsciousness (gold), Pre-RORR (Pink), and Post-RORR (green; n=8 animals). The fifth bar (orange) represents the Recovery epoch from the rats with extended recovery and was compared (one-tailed Wilcoxon text) with the Wake state from the same rats. (c) Representative spectrogram showing a significant decrease in power in higher gamma frequencies during ketamine-induced unconsciousness (Ketamine) and the appearance of high-frequency gamma power during emergence and after RORR. (d) Statistical comparisons (repeated measures anova) of changes in PSD between Wake (blue), Ketamine-induced unconsciousness (gold), Pre-RORR (Pink), and Post-RORR (green; n=8 animals). The fifth bar (orange) represents the Recovery epoch from the rats with extended recovery and was compared (one-tailed Wilcoxon text) with the Wake state from the same rats. The right axis applies only to the grey shaded area. *P<0.05 significant compared with Wake; #P<0.05 significant compared with Ketamine. Most of the comparisons are highly significant, but in order to avoid crowding the figure with the significance symbols, all the significance values are reported as P<0.05. The accurate P-values are reported in the text in the Results section.
British Journal of Anaesthesia  , DOI: ( /bja/aev095)
Copyright © 2015 The Author(s) Terms and Conditions

5. Fig. 4
Increase in ACh concentrations in the prefrontal cortex during ketamine-induced unconsciousness (Ketamine) and Pre- and Post-RORR (n=8 animals). Acetylcholine concentrations returned to the basal Wake concentrations after the prolonged recovery period. *P<0.05, **P<0.01, ***P<0.001 compared with Wake; #P<0.05, ##P<0.01 compared with Ketamine.
British Journal of Anaesthesia  , DOI: ( /bja/aev095)
Copyright © 2015 The Author(s) Terms and Conditions