1. PreBötC activity in low [K+]o implicates neuronal activity from both subpopulations.
PreBötC activity in low [K+]o implicates neuronal activity from both subpopulations. A, Voltage traces (top: average; middle: eupnea compartment; bottom: sigh compartment) of an in silico experiment in control conditions (left) and with VK set low (right). B, Schematic representation of the in vitro slice preparation from which an electrophysiological recording of preBötC activity was made simultaneously with calcium imaging performed on the contralateral respiratory network. Right, Image of a Ca2+-dye loaded slice obtained with a 40× objective. Yellow circles indicate rhythmically active cells. Bottom, Paired recordings of population electrical activity (top traces) and calcium transients (ΔF/F, bottom traces) in individual neurons (cells 1 to 19). All the cells displayed fluorescent changes synchronized to rhythmic electrical activity recorded on the contralateral side during both eupnea and sigh bursting in control conditions and during sigh-only activity in low [K+]o. Orange stars indicate sigh events.
Natalia Toporikova et al. eneuro 2015;2:ENEURO
©2015 by Society for Neuroscience